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v4 10x genomics pn 1000691 primescript rt master mix takara bio rr036a xf cell mito stress test kit agilent technologies  (TaKaRa)


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    TaKaRa v4 10x genomics pn 1000691 primescript rt master mix takara bio rr036a xf cell mito stress test kit agilent technologies
    V4 10x Genomics Pn 1000691 Primescript Rt Master Mix Takara Bio Rr036a Xf Cell Mito Stress Test Kit Agilent Technologies, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 27062 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/test+kit/PrimeScript+RT+Master+Mix/pm42887499-260-80-88
    Average 99 stars, based on 27062 article reviews
    v4 10x genomics pn 1000691 primescript rt master mix takara bio rr036a xf cell mito stress test kit agilent technologies - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Evaluation of BioFire Respiratory Panel 2 plus for Detection of Bordetella pertussis in Nasopharyngeal Swab Specimens from Children with Clinically Suspected Pertussis
    Article Snippet: .. Test kit: 10 × PCR buffer (Takara, Japan), 0.25 mM dNTP (Takara), 4 mM MgCl2, 0.5 μM of each amplification primer, PrimeScript TM II reverse transcriptase (Takara), TaKaRa Hot start-Taq polymerase, shrimp alkaline phosphatase (Zhejiang Digena Diagnostic Technology Co., Ltd.), MassARRAY chip (Zhejiang Digena Diagnostic Technology Co., Ltd.). .. Instrument and manufacturer: ABI 2720 PCR instrument (AB, USA), DP-TOF matrix-assisted laser desorption/ionization-time of flight mass spectrometry (Zhejiang Digena Diagnostic Technology Co., Ltd.), Typer 4.0 software (Zhejiang Digena Diagnostic Technology Co., Ltd.).

    Article Title: The Relationship Between Gene Polymorphism of Leptin and Leptin Receptor and Growth Hormone Deficiency
    Article Snippet: .. Both target genes were first amplified by PCR method using a test kit (Takara, Japan) using the parameters: pre-denaturing at 94°C for 2 min, followed by 40 cycles each of 94°C denaturing for 2 min, 63°C annealing for 30 s and 72°C elongation for 60 s. The reaction was repeated for 40 cycles using specifically designed primers ( ). ..

    Article Title: Comparison of extraction‐based and elution‐based polymerase chain reaction testing, and automated and rapid antigen testing for the diagnosis of severe acute respiratory syndrome coronavirus 2
    Article Snippet: We performed extraction‐based PCR using combinations of MP24 – LightMix (PCR condition 1), magLEAD – LightMix (PCR condition 2), and magLEAD – Takara (PCR condition 3). .. For elution‐based PCR, a clinical sample was directly mixed with sample processing reagents from Takara SARS‐CoV‐2 direct PCR detection kit (Takara Bio Inc.) and heated at 95°C for 5 min. After adding the PCR mixture to the test kit, we amplified it using COBAS® z480 (PCR condition 4). ..

    Amplification:

    Article Title: Evaluation of BioFire Respiratory Panel 2 plus for Detection of Bordetella pertussis in Nasopharyngeal Swab Specimens from Children with Clinically Suspected Pertussis
    Article Snippet: .. Test kit: 10 × PCR buffer (Takara, Japan), 0.25 mM dNTP (Takara), 4 mM MgCl2, 0.5 μM of each amplification primer, PrimeScript TM II reverse transcriptase (Takara), TaKaRa Hot start-Taq polymerase, shrimp alkaline phosphatase (Zhejiang Digena Diagnostic Technology Co., Ltd.), MassARRAY chip (Zhejiang Digena Diagnostic Technology Co., Ltd.). .. Instrument and manufacturer: ABI 2720 PCR instrument (AB, USA), DP-TOF matrix-assisted laser desorption/ionization-time of flight mass spectrometry (Zhejiang Digena Diagnostic Technology Co., Ltd.), Typer 4.0 software (Zhejiang Digena Diagnostic Technology Co., Ltd.).

    Article Title: The Relationship Between Gene Polymorphism of Leptin and Leptin Receptor and Growth Hormone Deficiency
    Article Snippet: .. Both target genes were first amplified by PCR method using a test kit (Takara, Japan) using the parameters: pre-denaturing at 94°C for 2 min, followed by 40 cycles each of 94°C denaturing for 2 min, 63°C annealing for 30 s and 72°C elongation for 60 s. The reaction was repeated for 40 cycles using specifically designed primers ( ). ..

    Article Title: Comparison of extraction‐based and elution‐based polymerase chain reaction testing, and automated and rapid antigen testing for the diagnosis of severe acute respiratory syndrome coronavirus 2
    Article Snippet: We performed extraction‐based PCR using combinations of MP24 – LightMix (PCR condition 1), magLEAD – LightMix (PCR condition 2), and magLEAD – Takara (PCR condition 3). .. For elution‐based PCR, a clinical sample was directly mixed with sample processing reagents from Takara SARS‐CoV‐2 direct PCR detection kit (Takara Bio Inc.) and heated at 95°C for 5 min. After adding the PCR mixture to the test kit, we amplified it using COBAS® z480 (PCR condition 4). ..

    Reverse Transcription:

    Article Title: Evaluation of BioFire Respiratory Panel 2 plus for Detection of Bordetella pertussis in Nasopharyngeal Swab Specimens from Children with Clinically Suspected Pertussis
    Article Snippet: .. Test kit: 10 × PCR buffer (Takara, Japan), 0.25 mM dNTP (Takara), 4 mM MgCl2, 0.5 μM of each amplification primer, PrimeScript TM II reverse transcriptase (Takara), TaKaRa Hot start-Taq polymerase, shrimp alkaline phosphatase (Zhejiang Digena Diagnostic Technology Co., Ltd.), MassARRAY chip (Zhejiang Digena Diagnostic Technology Co., Ltd.). .. Instrument and manufacturer: ABI 2720 PCR instrument (AB, USA), DP-TOF matrix-assisted laser desorption/ionization-time of flight mass spectrometry (Zhejiang Digena Diagnostic Technology Co., Ltd.), Typer 4.0 software (Zhejiang Digena Diagnostic Technology Co., Ltd.).

    Diagnostic Assay:

    Article Title: Evaluation of BioFire Respiratory Panel 2 plus for Detection of Bordetella pertussis in Nasopharyngeal Swab Specimens from Children with Clinically Suspected Pertussis
    Article Snippet: .. Test kit: 10 × PCR buffer (Takara, Japan), 0.25 mM dNTP (Takara), 4 mM MgCl2, 0.5 μM of each amplification primer, PrimeScript TM II reverse transcriptase (Takara), TaKaRa Hot start-Taq polymerase, shrimp alkaline phosphatase (Zhejiang Digena Diagnostic Technology Co., Ltd.), MassARRAY chip (Zhejiang Digena Diagnostic Technology Co., Ltd.). .. Instrument and manufacturer: ABI 2720 PCR instrument (AB, USA), DP-TOF matrix-assisted laser desorption/ionization-time of flight mass spectrometry (Zhejiang Digena Diagnostic Technology Co., Ltd.), Typer 4.0 software (Zhejiang Digena Diagnostic Technology Co., Ltd.).

    Quantitative RT-PCR:

    Article Title: Effects of a novel anti-biofilm peptide CRAMP combined with antibiotics on the formation of Pseudomonas aeruginosa biofilms.
    Article Snippet: The remarkable ability of Pseudomonas aeruginosa to form biofilms renders antibiotic treatments inefficient and therefore causing a wide variety of chronic infections.. The quorum sensing (QS) system in P. aeruginosa plays a role in the regulation of genes controlling virulence factors and biofilm formation, which may be an essential target for pharmacological intervention.. The present study aimed to investigate the synergistic activity of subMIC concentrations of CRAMP (a cathelicidin-related antimicrobial peptide) with fourteen antibiotics against P. aeroginusa biofilms.

    Activity Assay:

    Article Title: Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt
    Article Snippet: .. Tyrosine kinase (TK) inhibitory activity was determined using a commercial test kit (TK assay kit, nonradioactively, Takara Cat. #MK410). ..



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    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of <t>calcein</t> and Annexin V fluorescence results showing <t>mPTP</t> opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).
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    Image Search Results


    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Inhibition, CCK-8 Assay, Real-time Monitoring, Fluorescence, Flow Cytometry, Analysis, Staining

    Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Analysis, Quantitative RT-PCR, Western Blot, Staining, Fluorescence, Co-Immunoprecipitation Assay, Significance Assay